Axons are more sensitive to hydrogen peroxide than dendrites.A. Neurons were treated with 100 μM hydrogen peroxide for one hour, and then returned to control medium. At varying time points, they were fixed and stained with antibodies against MAP2 (a dendritic marker) and Tau (an axonal marker). Axons started degenerating at 3 h after treatment and mostly degenerated by 9 h (right panel). In contrast, dendrites of surviving cells remained intact even 24 h after treatment (left panel). Scale bar, 20 μm. B. Kymographs illustrating the transport of Golgi-derived vesicles in the axon and dendrite of the same neuron before and after one hour of hydrogen peroxide treatment. The transport of Golgi-derived vesicles was severely inhibited in dendrites as well as axons.
Fang et al. Molecular Neurodegeneration 2012 7:29 doi:10.1186/1750-1326-7-29