Table 3 |
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|
Targeted MS conditions for Septin11 peptides in gel band corresponding to 40-60 kDa. |
|||||||||||
|
1Precursor Ions (m/z) |
5Product Ions (m/z) |
6AUC (FTLD-U) |
AUC (Control) |
FTLD-U/Control |
|||||||
|
Protein Name |
Peptide Sequences |
|
|||||||||
|
Native (2L) |
3IS (4H) |
Name |
Native (L) |
IS (H) |
(L) |
(H) |
(L) |
(H) |
|||
|
|
|||||||||||
|
Septin11 |
FESDPATHNEPGVR |
778.4 |
783.4 |
||||||||
|
y2-10 |
539.4 |
544.4 |
2420 |
1034 |
1776 |
1531 |
2.0 |
||||
|
y1-10 |
1077.3 |
1087.3 |
5428 |
1176 |
3548 |
1797 |
2.3 |
||||
|
Septin11 |
AAAQLLQSQAQQSGAQQTK |
979.0 |
983.0 |
||||||||
|
y1-7 |
719.3 |
727.3 |
2700 |
790 |
1747 |
921 |
1.8 |
||||
|
y1-10 |
1046.4 |
1054.4 |
2242 |
743 |
1559 |
1030 |
2.0 |
||||
|
y1-12 |
1261.6 |
1269.6 |
3476 |
1074 |
1790 |
1334 |
2.4 |
||||
|
|
|||||||||||
|
2.1 ± 0.2 |
|||||||||||
|
Precursor Ions (m/z) |
7XIC (FTLD-U) |
XIC (Control) |
|||||||||
|
|
|
||||||||||
|
Native (L) |
IS (H) |
(L) |
(H) |
(L) |
(H) |
||||||
|
|
|||||||||||
|
β-actin |
AGFAGDDAPR |
488.73 |
493.74 |
3208 |
916 |
3799 |
717 |
0.7 |
|||
|
β-actin |
GYSFTTTAER |
566.77 |
571.78 |
2651 |
740 |
4109 |
896 |
0.8 |
|||
|
β-actin |
SYELPDGQVITIGNER |
895.96 |
900.96 |
2474 |
704 |
2806 |
584 |
0.7 |
|||
|
|
|||||||||||
|
0.7 ± 0.1 |
|||||||||||
|
|
|||||||||||
|
1The m/z values of native and labeled peptides 2Light 3Internal Standard 4Heavy 5The name and m/z values of monitored product ions (e.g. y1-7, the singly-charged y7 ion) 6Area under the curve 7Extracted ion chromatogram. |
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|
Gozal et al. Molecular Neurodegeneration 2011 6:82 doi:10.1186/1750-1326-6-82 |
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