Laser capture microdissection (LCM) of hippocampal neurons from Tg2576 mouse brain. Brain sections (10 μM) from Tg2576 transgenic mice along with nontransgenic controls were stained using HistoGene™ Stain, (Arcturus) to identify the neurons. (A) LCM was performed to selectively capture the neurons in CA1, CA3 and dentate gyrus regions onto Capsure LCM macro caps. Captured cells were processed for RNA isolation, RNA amplification and RT-PCR analysis. The mRNA levels of CREB (B) and its target BDNF (C) decreased in Tg2576 hippocampal neurons. *p < 0.01 compared to nontransgenic control.
Pugazhenthi et al. Molecular Neurodegeneration 2011 6:60 doi:10.1186/1750-1326-6-60