Additional file 4.

Target specificity of candidate siRNA molecules. (A) RNA preparations from cells treated for 24 h with 50 nM Mayo2 were analyzed by qRT-PCR. Although SNCA and SNCB diverge by only four bases within the Mayo2 sequence, silencing is specific to SNCA only. (B and C) Co-transfection studies in cells demonstrate that Mayo2 is active against both human and mouse SNCA, but human specific Mayo7 and Mayo8 do not silence mouse SNCA expression. Error bars = SEM, calculated from three independent assays.

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Lewis et al. Molecular Neurodegeneration 2008 3:19   doi:10.1186/1750-1326-3-19