Additional file 3.

Silencing of endogenous α-synuclein in vitro. (A) qRT-PCR of endogenous SNCA transcript from RNA preparations from cells treated with 50 nM siRNAs (Mayo 2, 7, 8, 9, siRNAMr) for 24 h. Each sample was assayed in quadruplicate, and expressed as a fold change from the untransfected control. * p < 0.05 t-test, Welch's modified t-test was used when variances differed. Error bars = SEM. (B) A typical immunoblot of cell extracts following 24 h transfection with 50 nM siRNA. The control is treated with transfection reagent alone. The position of a 16 kDa marker (lysozyme) is indicated. (C) Densitometric analysis of four independent experiments demonstrates significant reduction in the α-synuclein immunoreactivity (IR). * p < 0.05; ** p < 0.01 in t-test, Welch's modified t-test was used when variances differed. Error bars = SEM.

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Lewis et al. Molecular Neurodegeneration 2008 3:19   doi:10.1186/1750-1326-3-19