Figure 11.

Golgi fragmentation in Hup23 brainstem. A, Double immunofluorescence analysis of Hup23 brains stained with antibodies against p23 and GM130. Confocal images of neurons in pontine nuclei (upper panels) and a region in the middle of brainstem (lower panels) are shown. Arrowheads indicate abnormal p23 localization and Golgi fragmentation (visualized by GM130 staining) in neurons expressing high levels of p23. B, Immunoblot analysis of UPR markers in brainstem of Hup23 mice. Seventy five μg of total protein lysates of brainstem of Hup23 and non-transgenic littermates were fractionated on 4-20% SDS-PAGE gels and immunoblotted using antibodies against the indicated proteins. Hsc70 and GAPDH levels served as loading controls. Lysates from mouse embryonic fibroblasts (MEF) exposed overnight to 2 μg/ml tunicamycin (Tm) were analyzed on the same gels as positive control for the activation of the UPR.