Research article

Dantrolene is neuroprotective in Huntington's disease transgenic mouse model

Xi Chen¹, Jun Wu¹, Svetlana Lvovskaya^1,2, Emily Herndon³, Charlene Supnet^1,2 and Ilya Bezprozvanny^1,2*

• * Corresponding author: Ilya Bezprozvanny ilya.bezprozvanny@utsouthwestern.edu

Author Affiliations

¹ Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA

² Department of Medical Physics and Bioengineering, St Petersburg State Polytechnical University, St. Petersburg, Russia

³ Department of Pathology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA

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Molecular Neurodegeneration 2011, 6:81 doi:10.1186/1750-1326-6-81

Published: 25 November 2011

Abstract

Background

Huntington's disease (HD) is a progressive neurodegenerative disorder caused by a polyglutamine expansion in the Huntingtin protein which results in the selective degeneration of striatal medium spiny neurons (MSNs). Our group has previously demonstrated that calcium (Ca²⁺) signaling is abnormal in MSNs from the yeast artificial chromosome transgenic mouse model of HD (YAC128). Moreover, we demonstrated that deranged intracellular Ca²⁺ signaling sensitizes YAC128 MSNs to glutamate-induced excitotoxicity when compared to wild type (WT) MSNs. In previous studies we also observed abnormal neuronal Ca²⁺ signaling in neurons from spinocerebellar ataxia 2 (SCA2) and spinocerebellar ataxia 3 (SCA3) mouse models and demonstrated that treatment with dantrolene, a ryanodine receptor antagonist and clinically relevant Ca²⁺ signaling stabilizer, was neuroprotective in experiments with these mouse models. The aim of the current study was to evaluate potential beneficial effects of dantrolene in experiments with YAC128 HD mouse model.

Results

The application of caffeine and glutamate resulted in increased Ca²⁺ release from intracellular stores in YAC128 MSN cultures when compared to WT MSN cultures. Pre-treatment with dantrolene protected YAC128 MSNs from glutamate excitotoxicty, with an effective concentration of 100 nM and above. Feeding dantrolene (5 mg/kg) twice a week to YAC128 mice between 2 months and 11.5 months of age resulted in significantly improved performance in the beam-walking and gait-walking assays. Neuropathological analysis revealed that long-term dantrolene feeding to YAC128 mice significantly reduced the loss of NeuN-positive striatal neurons and reduced formation of Htt^exp nuclear aggregates.

Conclusions

Our results support the hypothesis that deranged Ca²⁺ signaling plays an important role in HD pathology. Our data also implicate the RyanRs as a potential therapeutic target for the treatment of HD and demonstrate that RyanR inhibitors and Ca²⁺ signaling stabilizers such as dantrolene should be considered as potential therapeutics for the treatment of HD and other polyQ-expansion disorders.