P-APP co-localization with MPM-2 at centrosomes in metaphase cells: Asynchronously growing (untreated, top row) and nocodazole arrested (bottom row) H4-15X cells were immunostained using the mitosis specific monoclonal antibody MPM2 and Thr668 P-APP polyclonal antibodies and staining was visualized using Alexa 488 and 594 fluorophores respectively. The untreated cells show P-APP localization in centrosomes in the mitotic cells. In the cells arrested with nocodazole the microtubules were completely depolymerized and P-APP showed significantly higher levels of amorphous staining. The nuclei were visualized using Hoechst stain. Magnification: 63×.
Judge et al. Molecular Neurodegeneration 2011 6:80 doi:10.1186/1750-1326-6-80