Figure 8.

Tau pathology in TDP-43_M337V mice. Immunohistochemistry of the cortices of (A) TDP-43_M337V and (B) TDP-43_WT mice showed elevated levels of phosphorylated tau (CP13) in both TDP-43_M337V and TDP-43_WT mice compared to (C) NT mice. (D) Western blotting of brain lysates from NT, TDP-43 _M337V and TDP-43_WT mice probed with CP13 antibody showed increases in murine tau phosphorylation at serine residues 202 in both lines of TDP-43 transgenic mice. Staining with tau-1 antibody showed reduced dephosphorylated tau levels in both lines of TDP-43 transgenic mice when compared to NT mice. Staining with antibody tau-5 showed that overall tau levels were equivalent across non-transgenic and transgenic mice. There was a dramatic increase of phospho-(Ser) PKC substrate in both TDP-43 transgenic lines indicating PKC activation that was not present in NT mice. Scale bars: 50 μM in A, B and C.