Flow cytometry analysis of blood monocyte subsets in WT, MyD88-/-, APPswe/PS1 and APPswe/PS1-MyD88+/- mice. Blood taken from the facial vein of 3-4 month-old mice was analyzed by FACS with the following fluorescently-labeled antibodies: CD45, CD11b, CD115 and Gr1. Leukocyte population is represented as cells in the CD45+ gate (a). To analyze exclusively monocytes in CD45+ cells, CD11bhi and CD115hi (CD45+CD11b+CD115+) cells were gated and the total percent for each group was quantified (b). No significant change in number of monocytes was revealed. Representatives histograms of monocytes for each group and their mean relative Gr1+ and Gr1- values (c) showed significantly less Gr1+ and more Gr1- monocytes in APPswe/PS1-MyD88+/- mice compare with APPswe/PS1 mice. This difference is even more noticeable while expressing the ratio of Gr1+ on Gr1- monocytes (d). Error bars: SEM; n = 3-5; *P < 0.05 (vs APPswe/PS1). (Statistical analysis was performed using one-way ANOVA followed by a Dunnett's or Tamhane's post-hoc test).
Michaud et al. Molecular Neurodegeneration 2011 6:5 doi:10.1186/1750-1326-6-5