Figure 5.

Overexpression of HA-tagged ATBF1 itself in primary cortical neurons did not induce apoptosis. TUNEL of primary cortical neurons transfected with HA-tagged ATBF1. The cells were transiently transfected with HA-tagged ATBF1. Twenty-four hours after transfection, TUNEL was performed, and then the cells were stained with the anti-HA antibody to detect transfected HA-ATBF1. Scale bars: 25 μm.