Three-dimensional reconstruction of cofilin-stained rods in deconvolved confocal image stacks from organotypic slices. Treatment of organotypic slices with Aβd/t results in a profound increase in cofilin-immunostained rods in the dentate gyrus/mossy fiber tract (DG/MFT) and a global change in cofilin distribution in cells in this region. (A) Single focal plane of non-rod-forming region near the CA3 compared to a rod hot spot in the dentate gyrus. Rods are evident in this single plane. (B) Three dimensional stack of planes from a cofilin stained control and Aβd/t-treated slice. Deconvolution of the confocal image stacks and thresholding the image by removing the lowest 20% of signal (lower panels) provides striking evidence of rod formation in this region. Hundreds of rods can be observed, which contain virtually all of the remaining immunostained cofilin.
Davis et al. Molecular Neurodegeneration 2011 6:10 doi:10.1186/1750-1326-6-10