Aβd/t fraction from 7PA2 cells, but not monomer, induces rods in dissociated hippocampal neurons. Analysis by fluorescence microscopy of dissociated neurons treated with vehicle (control), scrambled Aβ peptide (1 μM), or non-conditioned (NC media, d/t equivalent fraction), as well as with the monomer and d/t fractions from 7PA2 cell culture medium and 1 μM synthetic Aβ oligomers (Aβsyn). (A) Cofilin immunostained fluorescence images of hippocampal neurons showing representative responses with cofilin-actin rods (arrowheads) formed 24 h after treatment with Aβd/t and Aβsyn oligomers. Pretreatment of the Aβ-fractions with an antibody (6E10) to Aβ eliminates their rod-inducing effects. Bars = 10 μm. (B) Quantification of the rod forming response showing the neutralizing effects of the 6E10 antibody and the non-significant changes in rod formation by Aβ monomer. (* = p = 0.01 compared to control; # not significantly different from control).
Davis et al. Molecular Neurodegeneration 2011 6:10 doi:10.1186/1750-1326-6-10