Molecular Neurodegeneration

official impact factor 5.36
Search for
Advanced search
Molecular Neurodegeneration
Tools
Share this article
Open Access Research article

Axotomy-induced neurotrophic withdrawal causes the loss of phenotypic differentiation and downregulation of NGF signalling, but not death of septal cholinergic neurons

Oscar M Lazo1, Jocelyn C Mauna1,3, Claudia A Pissani1, Nibaldo C Inestrosa2 and Francisca C Bronfman1*

Author Affiliations

1 Department of Physiology, Neurobiology Unit, Center of Ageing and Regeneration (CARE), Nucleus Millennium in Regenerative Biology (MINREB), Faculty of Biological Sciences, Pontificia Universidad Catolica de Chile, Alameda 340, CP 8331010, Santiago, Chile

2 Department of Cellular Biology, Center of Ageing and Regeneration (CARE), Faculty of Biological Sciences, Pontificia Universidad Catolica de Chile, Alameda 340, CP 8331010, Santiago, Chile

3 Current address: Department of Neurobiology, University of Pittsburgh, Pittsburgh, PA, 15260, USA

For all author emails, please log on.

Molecular Neurodegeneration 2010, 5:5 doi:10.1186/1750-1326-5-5

Published: 19 January 2010

Additional files

Additional File 1:

Validation of p53 and activated-Caspase 3 as neurodegeneration markers in the septum. Confocal microscopy of coronal sections triple-labeled with anti-activated caspase-3, anti-p53 and Neurotrace (fluorescent Nissl stain) was performed in septa of rats injected with H2O2 (100 mM) or unlesioned control rats. The unlesioned brain shows no reactivity for p53 or activated-caspase 3, while in the H2O2-injected brain there are labeled neurons in the region of injection. Labeling neurons (Neurotrace-positive cells) with anti-p53 and anti-activated-caspase 3, validates these antibodies as neurodegeneration markers. Additional file: descriptions text (including details of how to view the file, if it is in a non-standard format).

Format: PDF Size: 878KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional File 2:

Neurotrace is a specific neuronal marker. Neurotrace is a specific neuronal stain and does not colocalize with glial markers. Left, confocal microscopy (scale bar: 500 μm) of sections double-labeled with Neurotrace and astroglia (top, anti-GFAP), microglia (center, anti-CD11B), oligodendroglia markers (bottom, anti-OMgp). Right panel shows inset magnifications (scale bar: 150 μm).

Format: PDF Size: 507KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data