Additional file 2.

Figure S2. Effects of experimental solution application to the measuring chamber on ion fluxes. An additional control was made to test a possible effect of solution disturbances in the measuring chamber on ion fluxes. Neurons at 14 DIV were used. Net K⁺ (A) and Ca²⁺ (B) fluxes were recorded continuously for 5 min (-5 to 0 min) and after vehicle (aCSF) application to the bath (0 to 10 min) with data acquired at a rate of 10 samples/sec and averaged over every 6 sec. Vehicle was applied at zero time as indicated by an arrow. No changes in K⁺ and Ca²⁺ fluxes were observed thus validating the approach used. Error bars are SEM (n = 4).

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Shabala et al. Molecular Neurodegeneration 2010 5:30   doi:10.1186/1750-1326-5-30