Research article
Prolonged Aβ treatment leads to impairment in the ability of primary cortical neurons to maintain K+ and Ca2+ homeostasis
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* Corresponding author: Lana Shabala L.Shabala@utas.edu.au
NeuroRepair Group, Menzies Research Institute, University of Tasmania. Private Bag 23, Hobart, Tasmania, 7001, Australia
Molecular Neurodegeneration 2010, 5:30 doi:10.1186/1750-1326-5-30
Published: 13 August 2010Additional files
Additional file 1:
Figure S1. Effects of acute application of Aβ1-40 on net K+ and Ca2+ fluxes. We tested whether acute application of Aβ1-40 to 14 DIV cortical neurons affected magnitudes of K+ and Ca2+ fluxes. Net fluxes of K+ (A) and Ca2+ (B) were recorded for 5 min (-5 to 0 min) followed by acute application of 1 μM Aβ1-40 (0 to 10 min) and 40 μM Aβ1-40 (final concentration, 10 to 25 min) to the bath. Neither concentration caused changes in net ion fluxes measured during the time course tested suggesting that prolonged treatment with Aβ1-40 is required to trigger disturbances in ion homeostasis. Error bars are SEM (n = 4).
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Additional file 2:
Figure S2. Effects of experimental solution application to the measuring chamber on ion fluxes. An additional control was made to test a possible effect of solution disturbances in the measuring chamber on ion fluxes. Neurons at 14 DIV were used. Net K+ (A) and Ca2+ (B) fluxes were recorded continuously for 5 min (-5 to 0 min) and after vehicle (aCSF) application to the bath (0 to 10 min) with data acquired at a rate of 10 samples/sec and averaged over every 6 sec. Vehicle was applied at zero time as indicated by an arrow. No changes in K+ and Ca2+ fluxes were observed thus validating the approach used. Error bars are SEM (n = 4).
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