Additional file 1:

(A), Time course of effect of Aβ_42-1 (gray circles) on fEPSPs peak amplitudes (n = 4). (B), Time course of effect of Aβ oligomers and Wnt-5a after (gray circles) on fEPSPs peak amplitudes (n = 3).

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Additional file 2:

Detection of Wnt-5a Ligand under action of Aβ oligomers. Neurons 15 DIV of culture were treated with Aβ oligomers for an hour and were immunostained for MAP2 protein and Wnt-5a ligand. (A), Show representative image to MAP, a: control neurons, b: Neurons treated with Aβ oligomers 50 nM, c: Neuron treated with Aβ 500 nM. Neuron was inmunostain with a specific antibody against Wnt-5a ligand, .d: Control Neurons, e: Neurons treated with Aβ oligomers 50 nM, f: Neuron treated with Aβ 500 nM, g: The graphs show fluorescence intensity for Wnt-5a ligand on neuron. Results are the mean ± S.E.M, in duplicate experiments, n = 3 separate experiments. Student's t-test *p < 0.050. (B), The soluble Wnt-5a ligand was detected in culture media for Hippocampal neurons 15 DIV by Sandwich ELISA technique under effect of Aβ oligomers.

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Additional file 3:

(A), Representative immunoblot of total synapsin-1 levels of neurons exposed to Aβ oligomers in the presence or absence of Wnt-5a and a quantification of densitometry are shown (n = 3). (B), Representative immunoblot of total HA-Wnt-5a levels of HEK-293 cells producing conditional medium.

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