PLA2 inhibitors protected cortical neurones against PrP82-146 induced synapse degeneration. (A) The synaptophysin content of cortical neurones pre-treated for with a vehicle control (■), 1 μM AACOCF3 (○), 1 μM MAFP (●), 5 μg/ml aristolochic acid (□), 10 μM U73122 (▲) or 10 μM ethyl-18-OCH3 (△) and subsequently incubated with varying concentrations of PrP82-146 for 24 hours. Values shown are the mean average amount of synaptophysin ± standard deviation (SD), n = 15. (B) Immunoblots showing the amount of synaptophysin and β-actin in cell extracts from neurones incubated with PrP82-146 (1.25 - 0.05 μM).
Bate et al. Molecular Neurodegeneration 2010 5:13 doi:10.1186/1750-1326-5-13