Table 2

Examination of Aβ values from conditioned media of HEK-293 derived stable cell lines expressing both Swedish APP and each of PS1 genes.

Cell Line
Transfected PS1 Constructs
Aβ1–40 (pg/ml)
Aβ1–42 (pg/ml)
Ratio of Aβ42/Aβ

H125.3-16
----
3152 ± 47 (n = 6)
337 ± 36 (n = 6)
0.097 ± 0.010
"
PS1
8379 ± 170 (n = 3)
1123 ± 20 (n = 6)
0.118 ± 0.002
"
PS1-M146V
9507 ± 129 (n = 3)
2627 ± 52 (n = 3)
0.217 ± 0.004**
"
PS1-D257TG
9716 ± 192 (n = 3)
1099 ± 35 (n = 6)
0.102 ± 0.003*
"
PS1-D385TG
7783 ± 225 (n = 3)
16647 ± 799 (n = 3)
0.681 ± 0.033**
"
PS1-D257TG/D385TG
5390 ± 218 (n = 3)
6941 ± 97 (n = 3)
0.563 ± 0.008**

n = 3 to 6 wells in one experiment. Aβ represents total Aβ.

*, p < 0.001, **, p < 0.0001 as compared to PS1 wt transfected cells

Carter et al. Molecular Neurodegeneration 2008 3:6   doi:10.1186/1750-1326-3-6