|
Transient transfection of various PS1 constructs in HEK-293 cells derived stable cell lines expressing APP carrying Swedish mutation (H125.3-16). |
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| Cell Line |
Transfected PS1 Constructs |
% change Aβ1–40 |
% change Aβ1–42 |
ratio of Aβ42/Aβ |
|
|
||||
| H125.3-16 |
pcDNA |
- |
- |
0.065 ± 0.004 |
| " |
PS1 |
-5.4 |
-8.6 |
0.063 ± 0.004 |
| " |
PS1-M146V |
-10.5 |
33.7 |
0.095 ± 0.004** |
| " |
PS1-D257TG |
-5.4 |
-3.6 |
0.060 ± 0.003 |
| " |
PS1-D385TG |
-2.7 |
233.7 |
0.193 ± 0.008* |
| " |
PS1-D257TG/D385TG |
-7.1 |
148.5 |
0.156 ± 0.001* |
|
The levels of Aβ 40 and 42 were measured 48 hours post transfection. ** p < 0.05 as compared to PS1 wt transfected cells N = 3 * p < 0.01 as compared to PS1 wt transfected cells Note: Due to the variation of absolute Aβ numbers in different experiments, only the comparative result to the cells transfected with pcDNA was used for comparison. | ||||
Carter et al. Molecular Neurodegeneration 2008 3:6 doi:10.1186/1750-1326-3-6 |
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