Figure 2.

Endogenous IDE is localized in DRMs of living cells. A- Living N2aWT cells (panel 1–3) and primary hippocampal neurons (panel 4–6) were stained to label DRMs (panel 1 and 4) and IDE (panel 2 and 5). Merged images showed partial co-localization (arrows) on the PM (panel 3 and 6). Scale bar, 5 μm (panel 1–3) and 20 μm (panel 4–6).B- Immunogold electron microscopy on cryosections of N2aSW cells showed clusters of gold particles at the PM (panel 1). A higher magnification of the section framed in panel 1 (panel 2) clearly indicated co-localization of gold particles of different size corresponding to flotillin-1 (black arrows, 6 nm size) and IDE (white arrows, 15 nm size). Scale bar, 50 nm.