Additional file 6.

Species specificity of mouse and human SNCA siRNA. (A) A typical immunoblot of total protein extracts from cells co-transfected with plasmids conferring expression of EGFP (V; vector) or EGFP-NACP (H = human α-synuclein; M = mouse α-synuclein) alone (control) or with 50 nM of either Mayo8S2 or Mayo 8S2M siRNA. A reprobe of the blot with α-tubulin antibody was used to equalize loading levels. (B) Densitometric analysis of three independent assays demonstrates that silencing of SNCA expression by Mayo8S2 is human specific, and by Mayo8S2M is mouse specific. p < 0.01, t-test, Welch's modified t-test was used when variances differed. Error bars = SEM. *

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Lewis et al. Molecular Neurodegeneration 2008 3:19   doi:10.1186/1750-1326-3-19