Figure 3.

p23 depletion has minor effects on overall protein secretion. A, N2aSwe cells transfected with siRNA were labeled with [³⁵S]met/cys for 15 min or 3 h. Aliquots of lysates (5 and 10 μl) were separated by SDS-PAGE and analyzed by phosphorimaging. B, 5, 10 and 20 μl of aliquots of conditioned media (CM) from cells labeled for 3 h were analyzed. In the panel on the right, the gray scale image is depicted in pseudocolor. Red and green circles indicate increase and decrease in the intensity of secreted polypeptides, respectively, in cells transfected with p23 siRNA relative to NS siRNA.