Figure 2.

Primers for Wld^s and β-tubulin have very similar R² values. Standard curves for the Wld^s and β-tubulin gene in a serially diluted (1 in 10) template genomic DNA sample from wild-type (C57Bl/6J). Efficient amplification was obtained for both sets of primers, as demonstrated by the slopes of linear regression of the standard curves, and good correlation coefficients. C_t is cycle threshold.