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Experimental design of the neuroprotection and regeneration study |
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| Protection Study |
NTN (n = 15) Mock (n = 15) PBS (n = 15) |
15 days |
6-OHDA lesion |
4.5 days or every month (n = 15 in each group) |
Rotation behavior and Sacrifice for IHC, HPLC |
|
| Regeneration Study |
6-OHDA lesion (n = 45) |
4 weeks |
Rotation Behavior |
NTN (n = 15) Mock (n = 15) PBS (n = 15) |
every month (n = 15 in each group) |
Rotation behavior and Sacrifice for IHC, HPLC |
|
NTN-c17.2, Mock- c17.2, and PBS were transplanted into the striatum by the conventional microinjection method. Subsequently, 6-hydroxydopamine (6-OHDA) was infused into the striatum or into the medial forebrain bundle (MFB) and the ventral tegmental area (VTA) in the neuroprotection study or regeneration study, respectively (see Materials and Methods). After transplantation, all the animals were analyzed for apomorphine-induced rotational behavior and then sacrificed for immunohistochemistry (IHC) and high-performance liquid chromatography (HPLC) measurements. | ||||||
Liu et al. Molecular Neurodegeneration 2007 2:19 doi:10.1186/1750-1326-2-19 |
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