Figure 1.

Electrophoretic analysis of genotyping PCR products. Genomic tail DNAs were prepared as detailed in the methods sections. 20 ng of the same DNA preparations of the four genotypes (KO male: y/- ; WT male: y/+; Heterozygote female: +/- ; WT female: +/+) were submitted to the different PCR amplification protocols detailed in the methods section, before loading on a 1.5% agarose gel.