Figure 5.

A. Characterization of Aβ levels in hippocampus by Aβ sandwich ELISA. 12 rats per treatment were used for biochemical and histological analyses. Naïve rats have very low levels of either Aβ species. AAV-BRI-Aβ40 injected animals display an increase in both RIPA- and FA-soluble Aβ40 at 3 months post-injection. There is a marked increase in FA-soluble (RIPA-insoluble) Aβ42 in AAV-BRI-Aβ42 animals at 3 months. B. Amyloid pathology in AAV-BRI-Aβ42 animals. Only BRI-Aβ42 animals showed diffuse plaque-like structures within the hippocampus following immunostaining with pan anti-Aβ 1–16 antibody. These deposits did not stain with thioflavin S or Congo Red (not shown). Magnification 100x.